COVID-19 DROPLET DIGITAL PCR (DDPCR) ASSAY

Bio-Rad has developed a COVID-19 ddPCR assay designed to be a powerful nucleic acid tool in the battle against COVID-19, with sensitivity and precision beyond that of qPCR assays.

The 2019-nCoV CDC ddPCR Triplex Probe Assay is a quantitative assay optimized for use on our QX200 and QXDx Droplet Digital PCR Systems using the 1-Step RT-ddPCR Advanced Kit for Probes and SARS-CoV-2 positive and negative standards. The 2019-nCoV CDC ddPCR Assay is currently for research use only (EUA pending for diagnostic use).

• High sensitivity and precision in low viral abundance samples

• Resistant to inhibition often seen in RT-PCR testing

• Single-well test – 3 sequences aligned to CDC markers: SARS-CoV-2 N1 and N2 genes, human RPP30 gene

• Available in 200 reactions (1,000 reactions coming soon)

DROPLET DIGITAL PCR SYSTEMS

Bio-Rad’s Droplet Digital PCR Systems provide ultrasensitive and absolute nucleic acid quantification. Droplet Digital PCR is particularly useful for low-abundance targets, targets in complex backgrounds, or monitoring subtle changes in target levels that cannot be detected with real-time PCR.

• QX200 AUTODG DROPLET DIGITAL PCR SYSTEM FOR RESEARCH (RUO)

  • Simplifies ddPCR workflow and ensures consistency

    1. Generates droplets for 96 ddPCR reactions in 45 minutes

    2. One AutoDG Instrument can supply 4–5 droplet readers

    3. HEPA-filtered enclosure reduces contamination risk